• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

INPP5E Monoclonal antibody

INPP5E Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B3D6

Cat no : 68602-1-Ig

Print datasheet

Synonyms

INPP5E, PPI5PIV

验证数据展示

  • WB analysis using 68602-1-Ig

    WB analysis using 68602-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68602-1-Ig (INPP5E antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68602-1-Ig

    WB analysis using 68602-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68602-1-Ig (INPP5E antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HaCaT using 68602-1-Ig

    WB analysis of HaCaT using 68602-1-Ig

    HaCaT cells were subjected to SDS PAGE followed by western blot with 68602-1-Ig (INPP5E antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inSH-SY5Y cells, HaCaT cells, HEK-293 cells, U-251 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68602-1-Ig targets INPP5E in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen INPP5E fusion protein Ag11959 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name inositol polyphosphate-5-phosphatase, 72 kDa
Synonyms INPP5E, PPI5PIV
Calculated Molecular Weight 644 aa, 70 kDa
Observed Molecular Weight72 kDa
GenBank Accession NumberBC028032
Gene Symbol INPP5E
Gene ID (NCBI) 56623
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRR6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215).

实验方案

Product Specific Protocols
WB protocol for INPP5E antibody 68602-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols