• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SHCBP1 Monoclonal antibody

SHCBP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B2E12

Cat No : 68647-1-Ig

Print datasheet

Synonyms

FLJ22009, PAL, SHCBP1

验证数据展示

  • WB analysis using 68647-1-Ig

    WB analysis using 68647-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68647-1-Ig (SHCBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 68647-1-Ig

    IHC staining of human liver cancer using 68647-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 68647-1-Ig

    IHC staining of human liver cancer using 68647-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inPC-3 cells, U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68647-1-Ig targets SHCBP1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen SHCBP1 fusion protein Ag17517 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SHC SH2-domain binding protein 1
Synonyms FLJ22009, PAL, SHCBP1
Calculated Molecular Weight 672 aa, 76 kDa
Observed Molecular Weight76 kDa
GenBank Accession NumberBC030699
Gene Symbol SHCBP1
Gene ID (NCBI) 79801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8NEM2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHCBP1 was initially identified as a potential gene associated with cell proliferation in fibroblasts. SHCBP1 exerts a crucial physiological function in normal tissue development. Chen et al. showed that SHCBP1 is an essential component of FGF signaling in neural progenitor cells. SHCBP1 is also upregulated during T cell proliferation and regulates CD4+ T cell effector function in vivo. Recently, SHCBP1 was shown to be closely associated with cancer development.

实验方案

Product Specific Protocols
WB protocol for SHCBP1 antibody 68647-1-IgDownload protocol
IHC protocol for SHCBP1 antibody 68647-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols