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NASP Monoclonal antibody

NASP Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A1

Cat no : 68710-1-Ig

Print datasheet

Synonyms

DKFZp547F162, FLB7527, FLJ31599, FLJ35510, NASP, PRO1999

验证数据展示

  • WB analysis using 68710-1-Ig

    WB analysis using 68710-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68710-1-Ig (NASP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, Ramos cells, U2OS cells
Planning an IHC experiment? We recommend our IHCeasy NASP Ready-To-Use IHC Kit. NASP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68710-1-Ig targets NASP in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen NASP fusion protein Ag1824 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear autoantigenic sperm protein (histone-binding)
Synonyms DKFZp547F162, FLB7527, FLJ31599, FLJ35510, NASP, PRO1999
Calculated Molecular Weight 788 aa, 85 kDa
Observed Molecular Weight150 kDa, 70 kDa
GenBank Accession NumberBC010105
Gene Symbol NASP
Gene ID (NCBI) 4678
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49321
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NASP (nuclear autoantigenic sperm protein) is associated with DNA replication, cell proliferation and cell cycle progression through functioning as a Histone H1 binding protein that mediates histone transport to the nucleus. NASP had two isoforms, tNASP (testis type) and sNASP (somatic type). The tNASP was mainly expressed in testis, a variety of malignant tumors, stem cells and embryonic tissues, while sNASP existed in all somatic mitosis cells. This antibody can recognize both isoforms.

实验方案

Product Specific Protocols
WB protocol for NASP antibody 68710-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols