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ADAM17 Monoclonal antibody

ADAM17 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F4C2

Cat no : 68725-1-Ig

Print datasheet

Synonyms

ADAM 17, ADAM17, CD156b, cSVP, Snake venom like protease, TACE, TNF alpha convertase, TNF alpha converting enzyme

验证数据展示

  • WB analysis using 68725-1-Ig

    WB analysis using 68725-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68725-1-Ig (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68725-1-Ig

    WB analysis using 68725-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68725-1-Ig (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inU2OS cells, NIH/3T3 cells, Calu-3 cells, A549 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68725-1-Ig targets ADAM17 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen ADAM17 fusion protein Ag34022 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ADAM metallopeptidase domain 17
Synonyms ADAM 17, ADAM17, CD156b, cSVP, Snake venom like protease, TACE, TNF alpha convertase, TNF alpha converting enzyme
Calculated Molecular Weight 824 aa, 93 kDa
Observed Molecular Weight 90-110 kDa
GenBank Accession NumberBC136783
Gene Symbol ADAM17
Gene ID (NCBI) 6868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP78536
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).

实验方案

Product Specific Protocols
WB protocol for ADAM17 antibody 68725-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols