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验证数据展示

  • WB analysis of TT using 68774-1-Ig

    WB analysis of TT using 68774-1-Ig

    TT cells were subjected to SDS PAGE followed by western blot with 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 68774-1-Ig

    IHC staining of human thyroid cancer using 68774-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human thyroid cancer using 68774-1-Ig

    IHC staining of human thyroid cancer using 68774-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CALCA/Calcitonin Monoclonal antibody

CALCA/Calcitonin Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68774-1-Ig

产品说明书

CloneNo. 1A12A1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA

别名

CALCA/CGRP, CALCA, Calcitonin, 1A12A1, CALC1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTT cells
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68774-1-Ig targets CALCA/Calcitonin in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 CALCA/Calcitonin fusion protein Ag26141 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Calcitonin
别名 CALCA/CGRP, CALCA, Calcitonin, 1A12A1, CALC1
计算分子量 141 aa, 15 kDa
观测分子量14 kDa
GenBank蛋白编号BC093753
基因名称 CALCA
Gene ID (NCBI) 796
RRIDAB_3670424
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01258
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calcitonin is a peptide hormone consisting of 32 amino acids, primarily synthesized by the parafollicular cells (C cells) of the human thyroid gland. It plays a crucial role in regulating calcium levels in the blood by decreasing them. Calcitonin opposes the actions of parathyroid hormone, which increases blood calcium levels. Calcitonin works through a G protein-coupled receptor, known as the calcitonin receptor, which predominantly transmits signals via the cAMP and PLC/IP3 pathways. Its primary physiological effects are on osteoclasts and the tubular epithelium of kidneys.

实验方案

Product Specific Protocols
WB protocol for CALCA/Calcitonin antibody 68774-1-IgDownload protocol
IHC protocol for CALCA/Calcitonin antibody 68774-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols