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DDAH1 Monoclonal antibody

DDAH1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A6

Cat no : 68823-1-Ig

Print datasheet

Synonyms

DDAH, DDAH 1, DDAH1, DDAHI, Dimethylargininase 1, FLJ21264, FLJ25539

验证数据展示

  • WB analysis using 68823-1-Ig

    WB analysis using 68823-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68823-1-Ig (DDAH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68823-1-Ig

    WB analysis using 68823-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68823-1-Ig (DDAH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inCalu-3 cells, SH-SY5Y cells, HT-29 cells, SK-N-SH cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68823-1-Ig targets DDAH1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen DDAH1 fusion protein Ag32840 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dimethylarginine dimethylaminohydrolase 1
Synonyms DDAH, DDAH 1, DDAH1, DDAHI, Dimethylargininase 1, FLJ21264, FLJ25539
Calculated Molecular Weight31kd
Observed Molecular Weight35 kDa
GenBank Accession NumberNM_012137
Gene Symbol DDAH1
Gene ID (NCBI) 23576
RRIDAB_3670434
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO94760
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dimethylarginine dimethylaminohydrolase 1 (DDAH1) is an enzyme that can degrade asymmetric dimethylarginine (ADMA), an endogenous nitric oxide synthase (NOS)inhibitor (PMID:26996393). About 80% of endogenous ADMA is metabolized by DDAH, principally the DDAH1 isoform (PMID:22460174). It was reported that DDAH1 is highly expressed in vascular endothelial cells in hearts (PMID:19917889). The observed molecular weight of DDAH1 is 35-40 kDa in the literature.

实验方案

Product Specific Protocols
WB protocol for DDAH1 antibody 68823-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols