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验证数据展示

  • WB analysis using 68824-1-Ig

    WB analysis using 68824-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68824-1-Ig (EIF2B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 68824-1-Ig

    WB analysis of NIH/3T3 using 68824-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68824-1-Ig (EIF2B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68824-1-Ig

    IF Staining of HeLa using 68824-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF2B1 antibody (68824-1-Ig, Clone: 1H6A3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

EIF2B1 Monoclonal antibody

EIF2B1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68824-1-Ig

产品说明书

CloneNo. 1H6A3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, pig

应用

WB, IF/ICC, ELISA

别名

EIF2BA, eIF2B GDP-GTP exchange factor subunit alpha, EIF2B, EIF 2Balpha, EIF 2B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, U2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68824-1-Ig targets EIF2B1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, pig
免疫原 EIF2B1 fusion protein Ag12493 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa
别名 EIF2BA, eIF2B GDP-GTP exchange factor subunit alpha, EIF2B, EIF 2Balpha, EIF 2B
计算分子量 305 aa, 34 kDa
观测分子量34 kDa
GenBank蛋白编号BC103763
基因名称 EIF2B1
Gene ID (NCBI) 1967
RRIDAB_3670435
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14232
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EIF2B1 antibody 68824-1-IgDownload protocol
IF protocol for EIF2B1 antibody 68824-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols