Mono/Di-Methyl-Histone H3 (Lys9) Monoclonal antibody
Mono/Di-Methyl-Histone H3 (Lys9) Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2E6D11
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, LNCaP cells, Jurkat cells, MOLT-4 cells, K-562 cells, NIH/3T3 cells, C2C12 cells, HSC-T6 cells |
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68825-1-Ig targets Mono/Di-Methyl-Histone H3 (Lys9) in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | Human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | histone cluster 1, H3a |
Synonyms | H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone cluster 1, H3a, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l |
Observed Molecular Weight | 17 kDa |
GenBank Accession Number | BC066245 |
Gene Symbol | HIST1H3A |
Gene ID (NCBI) | 8350 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P68431 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified. Methylation of Histone H3 at lysine 9 is linked to transcriptional repression. This antibody is specific to monomethyl or dimethyl-Histone H3 while it does not recognize trimethyl-Histone H3 (Lys9). It is also named as H3K9me1/2.
实验方案
Product Specific Protocols | |
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WB protocol for Mono/Di-Methyl-Histone H3 (Lys9) antibody 68825-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |