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MYO18A Monoclonal antibody

MYO18A Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A2B12

Cat no : 68868-1-Ig

Print datasheet

Synonyms

myosinXVIIIA, myosin XVIIIA, Molecule associated with JAK3 N-terminus, KIAA0216, CD245

验证数据展示

  • WB analysis using 68868-1-Ig

    WB analysis using 68868-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68868-1-Ig (MYO18A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHaCaT cells, A431 cells, SW 1990 cells, HT-29 cells, MDA-MB-468 cells, HeLa cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy MYO18A Ready-To-Use IHC Kit. MYO18A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68868-1-Ig targets MYO18A in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen MYO18A fusion protein Ag6185 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myosin XVIIIA
Synonyms myosinXVIIIA, myosin XVIIIA, Molecule associated with JAK3 N-terminus, KIAA0216, CD245
Calculated Molecular Weight 233 kDa
Observed Molecular Weight 230 kDa, 190 kDa
GenBank Accession NumberBC039612
Gene Symbol MYO18A
Gene ID (NCBI) 399687
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92614
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYO18A is an unconventional myosin that may play a role in the stabilization and organization of the actin cytoskeleton. Recently MYO18A has been identified as the receptor of SP and is responsible for clearance of SP-A-opsonized bacteria in in vivo infection. Two different isoforms of mouse Myo18A have been identified: Myo18A-alpha and Myo18A-beta as 230- and 190-kDa proteins, respectively. Myo18a-alpha colocalizes with the ER-Golgi complex and with membrane ruffles and filopodia, while Myo18a-beta localizes diffusely to the cytoplasm.

实验方案

Product Specific Protocols
WB protocol for MYO18A antibody 68868-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols