验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, Calu-3 cells, SW480 cells, LNCaP cells, HeLa cells, HEK-293 cells, SK-BR-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68898-3-Ig targets ICT1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | ICT1 fusion protein Ag33109 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | immature colon carcinoma transcript 1 |
| 别名 | Large ribosomal subunit protein mL62, EC:3.1.1.29, DS-1, DS1, 39S ribosomal protein L58, mitochondrial |
| 计算分子量 | 24 kDa |
| 观测分子量 | 21-24 kDa |
| GenBank蛋白编号 | BC015335 |
| 基因名称 | ICT1 |
| Gene ID (NCBI) | 3396 |
| RRID | AB_3670448 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q14197 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. One potential marker is immature colon carcinoma transcript 1 (ICT1, synonym: DS-1) whose mRNA and protein were more highly expressed in undifferentiated than differentiated cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ICT1 antibody 68898-3-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
