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验证数据展示

  • WB analysis of LNCaP using 68924-1-Ig

    WB analysis of LNCaP using 68924-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 68924-1-Ig

    WB analysis of MCF-7 using 68924-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 68924-1-Ig

    WB analysis of HEK-293 using 68924-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig liver using 68924-1-Ig

    WB analysis of pig liver using 68924-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ARF6 Monoclonal antibody

ARF6 Monoclonal Antibody for WB, ELISA
Cat No. 68924-1-Ig

产品说明书

CloneNo. 1H5H1

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, ELISA

别名

EC:3.6.5.2, ADP-ribosylation factor 6, ADP ribosylation factor 6, 1H5H1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, MCF-7 cells, pig liver tissue
Planning an IHC experiment? We recommend our IHCeasy ARF6 Ready-To-Use IHC Kit. ARF6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68924-1-Ig targets ARF6 in WB, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, pig
免疫原 ARF6 fusion protein Ag12642 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ADP-ribosylation factor 6
别名 EC:3.6.5.2, ADP-ribosylation factor 6, ADP ribosylation factor 6, 1H5H1
计算分子量 20 kDa
观测分子量 17-20 kDa
GenBank蛋白编号BC002952
基因名称 ARF6
Gene ID (NCBI) 382
RRIDAB_3670454
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP62330
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF6 is one of best identified ARFs It is present at the plasma membrane and to some extent on endosomal membranes where it regulates the flow of trafficking into and out of the cell and the actin cytoskeleton. The antibody is specific to ARF6.

实验方案

Product Specific Protocols
WB protocol for ARF6 antibody 68924-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols