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ARF6 Monoclonal antibody

ARF6 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H5H1

Cat no : 68924-1-Ig

Print datasheet

Synonyms

EC:3.6.5.2, ADP-ribosylation factor 6, ADP ribosylation factor 6, 1H5H1

验证数据展示

  • WB analysis of LNCaP using 68924-1-Ig

    WB analysis of LNCaP using 68924-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 68924-1-Ig

    WB analysis of MCF-7 using 68924-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 68924-1-Ig

    WB analysis of HEK-293 using 68924-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig liver using 68924-1-Ig

    WB analysis of pig liver using 68924-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, MCF-7 cells, pig liver tissue
Planning an IHC experiment? We recommend our IHCeasy ARF6 Ready-To-Use IHC Kit. ARF6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68924-1-Ig targets ARF6 in WB, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, pig
Immunogen ARF6 fusion protein Ag12642 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ADP-ribosylation factor 6
Synonyms EC:3.6.5.2, ADP-ribosylation factor 6, ADP ribosylation factor 6, 1H5H1
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 17-20 kDa
GenBank Accession NumberBC002952
Gene Symbol ARF6
Gene ID (NCBI) 382
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP62330
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF6 is one of best identified ARFs It is present at the plasma membrane and to some extent on endosomal membranes where it regulates the flow of trafficking into and out of the cell and the actin cytoskeleton. The antibody is specific to ARF6.

实验方案

Product Specific Protocols
WB protocol for ARF6 antibody 68924-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols