• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MLXIP Monoclonal antibody

MLXIP Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E11A4

Cat no : 68977-1-Ig

Print datasheet

Synonyms

MLX interacting protein, MIR, KIAA0867, Class E basic helix-loop-helix protein 36, BHLHE36

验证数据展示

  • WB analysis using 68977-1-Ig

    WB analysis using 68977-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68977-1-Ig (MLXIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inACHN cells, A549 cells, HEK-293 cells, Jurkat cells, K-562 cells, Mo7e cells
Planning an IHC experiment? We recommend our IHCeasy MLXIP Ready-To-Use IHC Kit. MLXIP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68977-1-Ig targets MLXIP in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen MLXIP fusion protein Ag4519 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name MLX interacting protein
Synonyms MLX interacting protein, MIR, KIAA0867, Class E basic helix-loop-helix protein 36, BHLHE36
Calculated Molecular Weight 919 aa, 100 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC039704
Gene Symbol MLXIP
Gene ID (NCBI) 22877
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HAP2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mlx-interacting protein(MLXIP), after forming heterodimers with MLX, can bind to and then activate transcription from CACGTG E boxes. The N terminus of MLXIP acts as a CRM1-dependent nuclear export signal and contribute to the cytoplasmic localization of the complex, and as a binding site for 13 protein. the C terminus contains a cytoplasmic localization signal and mediates protein-protein interaction of MONDOA and MLX. The complex play a role in transcriptional activation of glycolytic target genes and glucose-responsive gene regulation. This antibody could recognize isoform1(~110kd) and isoform3(~69kd) of MLXIP. A prominent and specific MLXIP of approximately 130 kDa was detected. It demonstrates that MLXIP associated specifically with Mlx in the cytoplasm (PMID: 11073985).

实验方案

Product Specific Protocols
WB protocol for MLXIP antibody 68977-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols