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NeutraKine® GM-CSF Monoclonal antibody, PBS Only (Capture)

NeutraKine® GM-CSF Monoclonal Antibody for Sandwich ELISA, Neutralization, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Sandwich ELISA, Neutralization, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E10E7

Cat no : 69003-1-PBS

Print datasheet

Synonyms

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, NeutraKine® GM-CSF, Sargramostim

验证数据展示

  • Neutralization experiment of NeutraKine® GM-CSF using 69003-1-Ig (same clone as 69003-1-PBS)

    Neutralization experiment of NeutraKine® GM-CSF using 69003-1-Ig (same clone as 69003-1-PBS)

    Recombinant human GM-CSF (Cat.NO. HZ-1002) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human GM-CSF (0.2 ng/mL HZ-1002) is neutralized by mouse anti-human GM-CSF monoclonal antibody 69003-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1.5-4 μg/mL This data was developed using the same antibody clone with 69003-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50067-1

    Sandwich ELISA standard curve of MP50067-1

    Sandwich ELISA standard curve of MP50067-1, GM-CSF Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 69003-1-PBS. Detection antibody: 68702-1-PBS. Standard: Eg0189. Range: 7.8-500 pg/mL.

产品信息

69003-1-PBS targets NeutraKine® GM-CSF as part of a matched antibody pair:

MP50067-1: 69003-1-PBS capture and 68702-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Sandwich ELISA, Neutralization, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen human Humankine GM-CSF protein HZ-1002 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name colony stimulating factor 2 (granulocyte-macrophage)
Synonyms Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, NeutraKine® GM-CSF, Sargramostim
Gene Symbol GM-CSF
Gene ID (NCBI) 1437
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein A purification
Storage Buffer PBS Only
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

This antibody can be used to neutralize the bioactivity of GM-CSF.