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AMPK Alpha Recombinant antibody

AMPK Alpha Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4N5

Cat No : 80209-5-RR

Print datasheet

Synonyms

AMPK, AMPK alpha, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1

验证数据展示

  • WB analysis using 80209-5-RR

    WB analysis using 80209-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80209-5-RR (AMPK alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity experiments of 80209-5-RR

    Affinity experiments of 80209-5-RR

    Biolayer interferometry (BLl) kinetic assays of 80209-5-RR against Human AMPK alpha were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy PRKAA1 Ready-To-Use IHC Kit. PRKAA1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80209-5-RR targets AMPK Alpha in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen AMPK Alpha fusion protein Ag1367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein kinase, AMP-activated, alpha 1 catalytic subunit
Synonyms AMPK, AMPK alpha, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC012622
Gene Symbol PRKAA1
Gene ID (NCBI) 5562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13131
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing. 80209-5-RR can recognize both AMPK Alpha 1 and AMPK Alpha 2.

实验方案

Product Specific Protocols
WB protocol for AMPK Alpha antibody 80209-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols