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验证数据展示

  • WB analysis using 81225-1-RR

    WB analysis using 81225-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81225-1-RR (GRB10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of LNCaP using 81225-1-RR

    IF Staining of LNCaP using 81225-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using GRB10 antibody (81225-1-RR, Clone: 1D13 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 81225-1-RR

    Affinity and Kinetic Characterization of 81225-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81225-1-RR against Human GRB10 were performed. The affinity constant is 0.195 nM.

GRB10 Recombinant antibody

GRB10 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 81225-1-RR

产品说明书

CloneNo. 1D13

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

KIAA0207, Insulin receptor-binding protein Grb-IR, Growth factor receptor-bound protein 10, GRBIR, 1D13

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, NIH/3T3 cells, HSC-T6 cells
Positive IF/ICC detected inLNCaP cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81225-1-RR targets GRB10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GRB10 fusion protein Ag20355 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 growth factor receptor-bound protein 10
别名 KIAA0207, Insulin receptor-binding protein Grb-IR, Growth factor receptor-bound protein 10, GRBIR, 1D13
计算分子量536aa,61 kDa; 594aa,67 kDa
观测分子量60-70 kDa
GenBank蛋白编号BC024285
基因名称 GRB10
Gene ID (NCBI) 2887
RRIDAB_2923710
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13322
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRB10 is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including INSR and IGF1R. It may play a role in mediating ubiquitination of INSR, leading to proteasomal degradation. GRB10 has 4 isoforms with the molecular mass of 60-70 kDa, which are produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for GRB10 antibody 81225-1-RRDownload protocol
IF protocol for GRB10 antibody 81225-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols