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GADD34 Recombinant antibody

GADD34 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4K3

Cat no : 81250-1-RR

Print datasheet

Synonyms

PPP1R15A, Growth arrest and DNA damage-inducible protein GADD34, growth arrest and DNA damage-inducible protein 34, 4K3

验证数据展示

  • WB analysis of HeLa using 81250-1-RR

    WB analysis of HeLa using 81250-1-RR

    MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 81250-1-RR (GADD34 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 81250-1-RR

    WB analysis of HepG2 using 81250-1-RR

    MG132 treated HepG2 cells were subjected to SDS PAGE followed by western blot with 81250-1-RR (GADD34 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 81250-1-RR

    IF Staining of PC-3 using 81250-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GADD34 antibody (81250-1-RR, Clone: 4K3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 81250-1-RR

    Affinity and Kinetic Characterization of 81250-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81250-1-RR against Human GADD34 were performed. The affinity constant is 93.8 pM.

经过测试的应用

Positive WB detected inMG132 treated HepG2 cells, MG132 treated NIH/3T3 cells, MG132 treated HeLa cells
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy PPP1R15A/GADD34 Ready-To-Use IHC Kit. PPP1R15A/GADD34 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81250-1-RR targets GADD34 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GADD34 fusion protein Ag0578 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 15A
Synonyms PPP1R15A, Growth arrest and DNA damage-inducible protein GADD34, growth arrest and DNA damage-inducible protein 34, 4K3
Calculated Molecular Weight 73 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC003067
Gene Symbol GADD34
Gene ID (NCBI) 23645
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75807
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4 (ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase (PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 is 100 kDa confirmed in GADD34 KO mice, and Proteintech's GADD34 antibody 81250-1-RR primarily recongizes the 100 kDa band.

实验方案

Product Specific Protocols
WB protocol for GADD34 antibody 81250-1-RRDownload protocol
IF protocol for GADD34 antibody 81250-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols