• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

Phospho-MNK1 (Thr250/255) Recombinant antibody

Phospho-MNK1 (Thr250/255) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4P9

Cat no : 81398-1-RR

Print datasheet

Synonyms

MKNK1, MNK1, Phospho-MKNK1 (Thr250/255), Phospho-MNK1 (Thr250/255)

验证数据展示

  • WB analysis of HEK-293T using 81398-1-RR

    WB analysis of HEK-293T using 81398-1-RR

    Non-treated HEK-293T cells, phosphatase inhibitor and λ phosphatase treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 81398-1-RR (Phospho-MNK1 (Thr250/255) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • WB analysis of HEK-293 using 81398-1-RR

    WB analysis of HEK-293 using 81398-1-RR

    WB result of Phospho-MNK1 (Thr250/255) antibody (81398-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Phospho-MNK1 (Thr250/255) transfected HEK-293 cells.

经过测试的应用

Positive WB detected inHEK-293T cells, HEK-293 cells, λ phosphatase treated HEK-293T cells

This antibody is equivalent to Thr197/202 in mice.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81398-1-RR targets Phospho-MNK1 (Thr250/255) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name MAP kinase interacting serine/threonine kinase 1
Synonyms MKNK1, MNK1, Phospho-MKNK1 (Thr250/255), Phospho-MNK1 (Thr250/255)
Calculated Molecular Weight 51 kDa
Observed Molecular Weight51 kDa
GenBank Accession NumberBC002755
Gene Symbol MNK1
Gene ID (NCBI) 8569
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BUB5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MNK1/MKNK1 (MAPK Interacting Serine/Threonine Kinase 1) is a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. MNK1 regulates diverse biologic processes including translation, cell proliferation, and differentiation through phosphorylation of different substrates(PMID: 29045529). Through phosphorylation MNK1 regulates the function of eukaryotic translation initiation factor 4E (eIF4E), a key player in translational control, whose expression is mostly upregulated in cancer patients.

实验方案

Product Specific Protocols
WB protocol for Phospho-MNK1 (Thr250/255) antibody 81398-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols