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验证数据展示

  • WB analysis of HEK-293T using 81398-1-RR

    WB analysis of HEK-293T using 81398-1-RR

    Non-treated HEK-293T cells, phosphatase inhibitor and λ phosphatase treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 81398-1-RR (Phospho-MNK1 (Thr250/255) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • WB analysis of HEK-293 using 81398-1-RR

    WB analysis of HEK-293 using 81398-1-RR

    WB result of Phospho-MNK1 (Thr250/255) antibody (81398-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Phospho-MNK1 (Thr250/255) transfected HEK-293 cells.

  • Featured Product
  • KD/KO验证

Phospho-MNK1 (Thr250/255) Recombinant antibody

Phospho-MNK1 (Thr250/255) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 81398-1-RR

产品说明书

CloneNo. 4P9

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

MKNK1, MNK1, Phospho-MKNK1 (Thr250/255), Phospho-MNK1 (Thr250/255)

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HEK-293 cells, λ phosphatase treated HEK-293T cells

This antibody is equivalent to Thr197/202 in mice.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81398-1-RR targets Phospho-MNK1 (Thr250/255) in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 MAP kinase interacting serine/threonine kinase 1
别名 MKNK1, MNK1, Phospho-MKNK1 (Thr250/255), Phospho-MNK1 (Thr250/255)
计算分子量 51 kDa
观测分子量51 kDa
GenBank蛋白编号BC002755
基因名称 MNK1
Gene ID (NCBI) 8569
RRIDAB_2935427
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BUB5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MNK1/MKNK1 (MAPK Interacting Serine/Threonine Kinase 1) is a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. MNK1 regulates diverse biologic processes including translation, cell proliferation, and differentiation through phosphorylation of different substrates(PMID: 29045529). Through phosphorylation MNK1 regulates the function of eukaryotic translation initiation factor 4E (eIF4E), a key player in translational control, whose expression is mostly upregulated in cancer patients.

实验方案

Product Specific Protocols
WB protocol for Phospho-MNK1 (Thr250/255) antibody 81398-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols