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验证数据展示

  • WB analysis using 81567-1-RR

    WB analysis using 81567-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81567-1-RR (FKBP10/FKBP65 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 81567-1-RR

    IHC staining of human ovary tumor using 81567-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81567-1-RR (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 81567-1-RR

    IHC staining of human ovary tumor using 81567-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81567-1-RR (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 81567-1-RR

    WB analysis of HeLa using 81567-1-RR

    WB result of FKBP10/FKBP65 antibody (81567-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP10/FKBP65 transfected HeLa cells.

  • Affinity and Kinetic Characterization of 81567-1-RR

    Affinity and Kinetic Characterization of 81567-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81567-1-RR against Human FKBP10/FKBP65 were performed. The affinity constant is 32.4 pM.

  • Featured Product
  • KD/KO验证

FKBP10/FKBP65 Recombinant antibody

FKBP10/FKBP65 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 81567-1-RR

产品说明书

CloneNo. S94

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

FKBP10, FKBP65, FKBP 65, FKBP 10, FK506-binding protein 10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, A375 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:11800
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81567-1-RR targets FKBP10/FKBP65 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 FKBP10/FKBP65 fusion protein Ag2814 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 FK506 binding protein 10, 65 kDa
别名 FKBP10, FKBP65, FKBP 65, FKBP 10, FK506-binding protein 10
计算分子量 582 aa, 64 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC016467
基因名称 FKBP10
Gene ID (NCBI) 60681
RRIDAB_2935562
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96AY3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FKBP10, also known as FKBP65, is a 65 kDa FK506 binding protein that is a member of the FKBP-type peptidyl-prolyl cis/trans isomerase family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone and binding partner of type I collagen. FKBP65 is expressed in developing tissues and re-expressed in adult tissues following injury. As documented, FKBP10 has been detected to play important roles in the initiation and progression of cancers.

实验方案

Product Specific Protocols
WB protocol for FKBP10/FKBP65 antibody 81567-1-RRDownload protocol
IHC protocol for FKBP10/FKBP65 antibody 81567-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols