Galectin 3 Recombinant antibody
Galectin 3 Recombinant Antibody for WB, IP, IF/ICC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (1)
Applications
WB, IP, IF/ICC, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
4M10
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, MCF-7 cells, A549 cells, A431 cells, NIH/3T3 cells |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF) | IF : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
82024-1-RR targets Galectin 3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.
Tested Applications | WB, IP, IF/ICC, IHC, ELISA Application Description |
Cited Applications | IHC |
Tested Reactivity | Human, mouse |
Cited Reactivity | human |
Immunogen | Galectin 3 fusion protein Ag6891 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | lectin, galactoside-binding, soluble, 3 |
Synonyms | 35 kDa lectin, CBP 35, CBP35, Gal 3, GAL3, Galactose specific lectin 3, Galactoside binding protein, GALBP, Galectin 3, GALIG, IgE binding protein, L 31, Laminin binding protein, Lectin L 29, LGALS2, LGALS3, Mac 2 antigen, MAC2 |
Calculated Molecular Weight | 26 kDa |
Observed Molecular Weight | 31 kDa |
GenBank Accession Number | BC001120 |
Gene Symbol | LGALS3 |
Gene ID (NCBI) | 3958 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P17931 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Galectin 3 antibody 82024-1-RR | Download protocol |
IHC protocol for Galectin 3 antibody 82024-1-RR | Download protocol |
IF protocol for Galectin 3 antibody 82024-1-RR | Download protocol |
IP protocol for Galectin 3 antibody 82024-1-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
JCI Insight HEY1-NCOA2 expression modulates chondrogenic differentiation and induces mesenchymal chondrosarcoma in mice |