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CD9 Recombinant antibody

CD9 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5K14

Cat no : 82105-1-RR

Print datasheet

Synonyms

CD9 antigen, BTCC 1, BA2, 5K14, 5H9 antigen

验证数据展示

  • WB analysis using 82105-1-RR

    WB analysis using 82105-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82105-1-RR (CD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 82105-1-RR

    IHC staining of human lung cancer using 82105-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD9 antibody included
  • IHC staining of human lung cancer using 82105-1-RR

    IHC staining of human lung cancer using 82105-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD9 antibody included
  • IF Staining of MCF-7 using 82105-1-RR

    IF Staining of MCF-7 using 82105-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (82105-1-RR, Clone: 5K14 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of human urine exosomes using 82105-1-RR

    WB analysis of human urine exosomes using 82105-1-RR

    human urine and human urine-derived exosomes were subjected to SDS PAGE followed by western blot with 82105-1-RR (CD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82105-1-RR

    Affinity and Kinetic Characterization of 82105-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82105-1-RR against Human CD9 were performed. The affinity constant is 36.3 pM.

经过测试的应用

Positive WB detected inHeLa cells, human urine exosomes tissue, L02 cells, A431 cells
Positive IHC detected inhuman lung cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CD9 Ready-To-Use IHC Kit. CD9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:650-1:2600
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82105-1-RR targets CD9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CD9 fusion protein Ag14546 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD9 molecule
Synonyms CD9 antigen, BTCC 1, BA2, 5K14, 5H9 antigen
Calculated Molecular Weight 228 aa, 25 kDa
Observed Molecular Weight23-27 kDa
GenBank Accession NumberBC011988
Gene Symbol CD9
Gene ID (NCBI) 928
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21926
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and support myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in fertilization of mammals (PMID: 25536312).

实验方案

Product Specific Protocols
WB protocol for CD9 antibody 82105-1-RRDownload protocol
IHC protocol for CD9 antibody 82105-1-RRDownload protocol
IF protocol for CD9 antibody 82105-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols