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ATP5A1 Recombinant antibody

ATP5A1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4O12

Cat no : 82288-1-RR

Print datasheet

Synonyms

ATP5A 1, ATP5A, ATP synthase F1 subunit alpha, 4O12

验证数据展示

  • WB analysis using 82288-1-RR

    WB analysis using 82288-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82288-1-RR (ATP5A1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82288-1-RR

    IF Staining of HepG2 using 82288-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of mouse brain using 82288-1-RR

    IHC staining of mouse brain using 82288-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82288-1-RR (ATP5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP5A1 antibody included
  • IHC staining of mouse brain using 82288-1-RR

    IHC staining of mouse brain using 82288-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82288-1-RR (ATP5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP5A1 antibody included
  • FC experiment of HeLa using 82288-1-RR

    FC experiment of HeLa using 82288-1-RR

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATP5A1 (82288-1-RR, Clone:4O12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inmouse brain tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ATP5A1 Ready-To-Use IHC Kit. ATP5A1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:1100-1:4400
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82288-1-RR targets ATP5A1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen ATP5A1 fusion protein Ag6385 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Synonyms ATP5A 1, ATP5A, ATP synthase F1 subunit alpha, 4O12
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC064562
Gene Symbol ATP5A1
Gene ID (NCBI) 498
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25705
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo-containing the membrane proton channel. F0 domain has 10 subunits: a,b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.

实验方案

Product Specific Protocols
WB protocol for ATP5A1 antibody 82288-1-RRDownload protocol
IHC protocol for ATP5A1 antibody 82288-1-RRDownload protocol
IF protocol for ATP5A1 antibody 82288-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols