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PTRF Recombinant antibody

PTRF Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3E15

Cat no : 82697-1-RR

Print datasheet

Synonyms

FKSG13, Cavin-1, Cavin 1, 3E15

验证数据展示

  • WB analysis using 82697-1-RR

    WB analysis using 82697-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82697-1-RR (PTRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82697-1-RR

    IF Staining of HepG2 using 82697-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTRF antibody (82697-1-RR, Clone: 3E15 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human stomach using 82697-1-RR

    IHC staining of human stomach using 82697-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82697-1-RR (PTRF antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTRF antibody included
  • Affinity and Kinetic Characterization of 82697-1-RR

    Affinity and Kinetic Characterization of 82697-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82697-1-RR against Human PTRF were performed. The affinity constant is 0.303 nM.

经过测试的应用

Positive WB detected inmouse lung tissue, rat lung tissue, mouse heart tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PTRF Ready-To-Use IHC Kit. PTRF primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82697-1-RR targets PTRF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PTRF fusion protein Ag13486 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name polymerase I and transcript release factor
Synonyms FKSG13, Cavin-1, Cavin 1, 3E15
Calculated Molecular Weight 43 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC008849
Gene Symbol PTRF
Gene ID (NCBI) 284119
RRIDAB_3086511
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6NZI2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PTRF antibody 82697-1-RRDownload protocol
IHC protocol for PTRF antibody 82697-1-RRDownload protocol
IF protocol for PTRF antibody 82697-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols