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验证数据展示

  • WB analysis using 82738-4-RR

    WB analysis using 82738-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82738-4-RR (TOP2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 82738-4-RR

    IP experiment of HeLa using 82738-4-RR

    IP result of anti-TOP2A (IP:82738-4-RR, 4ug; Detection:82738-4-RR 1:2000) with HeLa cells lysate 1280 ug.

  • IF Staining of A431 using 82738-4-RR

    IF Staining of A431 using 82738-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TOP2A antibody (82738-4-RR, Clone: 1J18 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 82738-4-RR

    Affinity and Kinetic Characterization of 82738-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82738-4-RR against Human TOP2A were performed. The affinity constant is 0.928 nM.

TOP2A Recombinant antibody

TOP2A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 82738-4-RR

产品说明书

CloneNo. 1J18

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

TOP2, TOP II, EC:5.6.2.2, DNA topoisomerase 2-alpha, DNA topoisomerase 2 alpha

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, HEK-293 cells, A431 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82738-4-RR targets TOP2A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 TOP2A fusion protein Ag19551 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 topoisomerase (DNA) II alpha 170kDa
别名 TOP2, TOP II, EC:5.6.2.2, DNA topoisomerase 2-alpha, DNA topoisomerase 2 alpha
计算分子量 1531 aa, 174 kDa
观测分子量~170 kDa
GenBank蛋白编号BC140791
基因名称 TOP2A
Gene ID (NCBI) 7153
RRIDAB_3086521
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11388
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

实验方案

Product Specific Protocols
WB protocol for TOP2A antibody 82738-4-RRDownload protocol
IF protocol for TOP2A antibody 82738-4-RRDownload protocol
IP protocol for TOP2A antibody 82738-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols