验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, HEK-293T cells, SH-SY5Y cells, Caco-2 cells, mouse brain tissue, rat brain tissue |
| Positive FC (Intra) detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82744-2-RR targets ADAMTS4 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ADAMTS4 fusion protein Ag2443 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ADAM metallopeptidase with thrombospondin type 1 motif, 4 |
| 别名 | ADAM-TS 4, ADAMTS 4, ADAM TS4, ADAM TS 4, A disintegrin and metalloproteinase with thrombospondin motifs 4 |
| 计算分子量 | 837 aa, 90 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC030812 |
| 基因名称 | ADAMTS4 |
| Gene ID (NCBI) | 9507 |
| RRID | AB_3670543 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O75173 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The ADAMTS (a disintegrin-like and metalloproteinase with thrombospondin motifs) is a family of extracellular metalloproteinases mediating diverse functions including matrix degradation, blood coagulation and angiogenesis. The ADAMTS family constitutes a group of proteins composed of 19 enzymes and 7 ADAMTS-like proteins. ADAMTS4 is a well-known proteoglycanase and has angiomodulatory properties. The 837 amino acid long human ADAMTS4 protein contains a long signal peptide, a prodomain, a metalloproteinase catalytic domain with zinc-binding motif, a disintegrin-like domain, a central TSR motif, followed by a cysteine-rich region with ten conserved cysteine residues, and a spacer domain. The full-length proform human ADAMTS4 (zymogen form) has been described to be 90 kDa. In extracellular matrix (ECM), ADAMTS4 undergoes further C-terminal cleavage at Lys694-Phe695 and Thr581-Phe582, respectively, to generate two other truncated forms-53 and 40 kDa (PMID:12202483).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAMTS4 antibody 82744-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |