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验证数据展示

  • WB analysis using 82798-3-RR

    WB analysis using 82798-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82798-3-RR (HNRNPC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 82798-3-RR

    IHC staining of human stomach using 82798-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82798-3-RR (HNRNPC antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HNRNPC antibody included
  • IF Staining of HeLa using 82798-3-RR

    IF Staining of HeLa using 82798-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPC antibody (82798-3-RR, Clone: 4F16 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). CoraLite® Plus 488-Phalloidin (green).

  • Affinity and Kinetic Characterization of 82798-3-RR

    Affinity and Kinetic Characterization of 82798-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82798-3-RR against Human HNRNPC were performed. The affinity constant is below 1 pM.

HNRNPC Recombinant antibody

HNRNPC Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 82798-3-RR

产品说明书

CloneNo. 4F16

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

HNRPC, hnRNP C1/C2, HNRNP, C2, C1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inhuman stomach tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HNRNPC Ready-To-Use IHC Kit. HNRNPC primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82798-3-RR targets HNRNPC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HNRNPC fusion protein Ag2356 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein C (C1/C2)
别名 HNRPC, hnRNP C1/C2, HNRNP, C2, C1
计算分子量 32 kDa
观测分子量 36-40 kDa
GenBank蛋白编号BC003394
基因名称 HNRNPC
Gene ID (NCBI) 3183
RRIDAB_3086542
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07910
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HNRNPC antibody 82798-3-RRDownload protocol
IHC protocol for HNRNPC antibody 82798-3-RRDownload protocol
IF protocol for HNRNPC antibody 82798-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols