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验证数据展示

  • WB analysis using 82806-7-RR

    WB analysis using 82806-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82806-7-RR (OLIG2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82806-7-RR

    Affinity and Kinetic Characterization of 82806-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82806-7-RR against Human OLIG2 were performed. The affinity constant is below 1 pM.

OLIG2 Recombinant antibody

OLIG2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 82806-7-RR

产品说明书

CloneNo. 240839C1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Class E basic helix-loop-helix protein 19, Class B basic helix-loop-helix protein 1, bHLHe19, bHLHb1, 5O7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, mouse brain tissue, rat brain tissue
Planning an IHC experiment? We recommend our IHCeasy OLIG2 Ready-To-Use IHC Kit. OLIG2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82806-7-RR targets OLIG2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 OLIG2 fusion protein Ag5089 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 oligodendrocyte lineage transcription factor 2
别名 Class E basic helix-loop-helix protein 19, Class B basic helix-loop-helix protein 1, bHLHe19, bHLHb1, 5O7
计算分子量 32 kDa
观测分子量35 kDa
GenBank蛋白编号BC047511
基因名称 OLIG2
Gene ID (NCBI) 10215
RRIDAB_3670559
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13516
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OLIG2, also named as BHLHB1, BHLHE19, PRKCBP2 and RACK17, is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1, OLIG2 establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. OLIG2 is widely expressed in subsets of glia cells and progenitors, and it is strongly induced at different sites by both acute and chronic injury, albeit with different mechanisms. OLIG2 acts as a repressor of neurogenesis in cells reacting to brain injury. It may represent an effective approach towards evoking neuronal repair from parenchymal precursors.(PMID:19390819 )

实验方案

Product Specific Protocols
WB protocol for OLIG2 antibody 82806-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols