TMEM27 Recombinant antibody
TMEM27 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240437C2
验证数据展示
经过测试的应用
Positive WB detected in | mouse kidney tissue, rat kidney tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 82853-2-RR is TMEM27 Fusion Protein expressed in E. coli.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human |
Immunogen | TMEM27 fusion protein Ag7668 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | transmembrane protein 27 |
Synonyms | NX17, NX 17, Collectrin, CLTRN, ACE2 |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 35-40 kDa |
GenBank Accession Number | BC050606 |
Gene Symbol | TMEM27 |
Gene ID (NCBI) | 57393 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q9HBJ8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMEM27 (also termed collectrin), a 46 kDa type I transmembrane protein, is a homolog of the noncatalytic domain of angiotensin-converting enzyme-related carboxypeptidase (Ace2). Its expression has only been reported in the brush border membrane of the proximal tubules and collecting ducts of the kidney and in the pancreatic b cell (PMID: 11278314, 16330324). Overexpression of TMEM27 in b cells leads to increased proliferation in vitro and increased pancreatic b cell mass in vivo, and also has been reported to augment glucosestimulated ins secretion (GSIS) (PMID: 16330324, 16330323). The abundance of TMEM27 protein in b cells is furthermore regulated by ectodomain cleavage, which leads to two cleavage products, a 25 kDa N-terminal part that is released into the extracellular space (the shed fragment), and a 22 kDa C-terminal fragment (CTF) remaining in the membrane that is rapidly degraded (PMID: 16330324). TMEM27 is an N-glycosylated protein and can form dimers with MW of 64-75 kDa(patent US 20100119489 A1). TMEM27 can be used as a beta cell mass biomarker (PMID: 20386877, 21907142).
实验方案
Product Specific Protocols | |
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WB protocol for TMEM27 antibody 82853-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |