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验证数据展示

  • FC experiment of U2OS using 82877-2-RR

    FC experiment of U2OS using 82877-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug APOL6 Recombinant antibody (82877-2-RR, Clone:230162E11)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of HeLa using 82877-2-RR

    IF Staining of HeLa using 82877-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using APOL6 antibody (82877-2-RR, Clone: 230162E11 ) at dilution of 1:100 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Affinity and Kinetic Characterization of 82877-2-RR

    Affinity and Kinetic Characterization of 82877-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82877-2-RR against Human APOL6 were performed. The affinity constant is 13.8 nM.

APOL6 Recombinant monoclonal antibody

APOL6 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 82877-2-RR

产品说明书

CloneNo. 230162E11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

230162E11, APOL 6, APOL VI, Apolipoprotein L-VI, APOLVI

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82877-2-RR targets APOL6 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag24430

Product name: Recombinant human APOL6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-36 aa of BC038950

Sequence: MDNQAERESEAGVGLQRDEDDAPLCEDVELQDGDLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 apolipoprotein L, 6
别名 230162E11, APOL 6, APOL VI, Apolipoprotein L-VI, APOLVI
计算分子量 38 kDa
GenBank蛋白编号BC038950
基因名称 APOL6
Gene ID (NCBI) 80830
RRIDAB_3670596
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BWW8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for APOL6 antibody 82877-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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