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验证数据展示

  • FC experiment of MCF-7 using 82893-1-RR

    FC experiment of MCF-7 using 82893-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human MELK (82893-1-RR, Clone:230106D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82893-1-RR

    Affinity and Kinetic Characterization of 82893-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82893-1-RR against Human MELK were performed. The affinity constant is below 1 pM.

MELK Recombinant antibody

MELK Uni-rAbTM Recombinant Antibody for FC (Intra), ELISA
Cat No. 82893-1-RR

产品说明书

CloneNo. 230106D4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra), ELISA

别名

KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy MELK Ready-To-Use IHC Kit. MELK primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82893-1-RR targets MELK in FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 MELK fusion protein Ag1897 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 maternal embryonic leucine zipper kinase
别名 KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2
计算分子量 651 aa, 75 kDa
GenBank蛋白编号BC014039
基因名称 MELK
Gene ID (NCBI) 9833
RRIDAB_3670618
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14680
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.

实验方案

Product Specific Protocols
FC protocol for MELK antibody 82893-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols