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MELK Recombinant antibody

MELK Uni-rAbTM Recombinant Antibody for FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230106D4

Cat no : 82893-1-RR

Print datasheet

Synonyms

KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2

验证数据展示

  • FC experiment of MCF-7 using 82893-1-RR

    FC experiment of MCF-7 using 82893-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human MELK (82893-1-RR, Clone:230106D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82893-1-RR

    Affinity and Kinetic Characterization of 82893-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82893-1-RR against Human MELK were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82893-1-RR targets MELK in FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen MELK fusion protein Ag1897 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name maternal embryonic leucine zipper kinase
Synonyms KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2
Calculated Molecular Weight 651 aa, 75 kDa
GenBank Accession NumberBC014039
Gene Symbol MELK
Gene ID (NCBI) 9833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14680
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.

实验方案

Product Specific Protocols
FC protocol for MELK antibody 82893-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols