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HLA-DMA Recombinant antibody, PBS Only (Detector)

HLA-DMA Recombinant Antibody for FC, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

230155D3

Cat No : 82899-1-PBS

Print datasheet

Synonyms

D6S222E, DAMA 147C13.1 001, DAMC 226H3.5 001, DAQB 96B10.1 001, DASS 67H14.8 001, DMA, DMA protein, hCG_2001572, HLA DMA, HLA DMA protein, HLADM, HLA-DMA, RING6, XXbac BCX136A3.1 001, XXbac BPG181M17.3 001

验证数据展示

  • FC experiment of A431 using 82899-1-RR (same clone as 82899-1-PBS)

    FC experiment of A431 using 82899-1-RR (same clone as 82899-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.13 ug Anti-Human HLA-DMA (82899-1-RR, Clone:230155D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.13 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82899-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00018-1

    Cytometric bead array standard curve of MP00018-1

    Cytometric bead array standard curve ofMP00018-1, HLA-DMA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82899-5-PBS. Detection antibody: 82899-1-PBS. Standard: Ag2452. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00018-3

    Cytometric bead array standard curve of MP00018-3

    Cytometric bead array standard curve ofMP00018-3, HLA-DMA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82899-3-PBS. Detection antibody: 82899-1-PBS. Standard: Ag2452. Range: 0.625-20 ng/mL.

82899-1-RR

产品信息

82899-1-PBS targets HLA-DMA as part of a matched antibody pair:

MP00018-1: 82899-5-PBS capture and 82899-1-PBS detection (validated in Cytometric bead array)

MP00018-3: 82899-3-PBS capture and 82899-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications FC, Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity Human
Immunogen HLA-DMA fusion protein Ag2452 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name major histocompatibility complex, class II, DM alpha
Synonyms D6S222E, DAMA 147C13.1 001, DAMC 226H3.5 001, DAQB 96B10.1 001, DASS 67H14.8 001, DMA, DMA protein, hCG_2001572, HLA DMA, HLA DMA protein, HLADM, HLA-DMA, RING6, XXbac BCX136A3.1 001, XXbac BPG181M17.3 001
Calculated Molecular Weight 261 aa, 29 kDa
GenBank Accession NumberBC026279
Gene Symbol HLA-DMA
Gene ID (NCBI) 3108
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP28067
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.