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AURKA Recombinant antibody

AURKA Recombinant Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

230087F7

Cat No : 82906-2-RR

Print datasheet

Synonyms

AIK, ARK 1, ARK1, AURA, AURKA, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7

验证数据展示

  • WB analysis using 82906-2-RR

    WB analysis using 82906-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82906-2-RR (AURKA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82906-2-RR

    IF Staining of HepG2 using 82906-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AURKA antibody (82906-2-RR, Clone: 230087F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells, HeLa cells, NCCIT cells
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy AURKA Ready-To-Use IHC Kit. AURKA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82906-2-RR targets AURKA in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, WB, ELISA Application Description
Tested Reactivity Human
Immunogen AURKA fusion protein Ag0362 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name aurora kinase A
Synonyms AIK, ARK 1, ARK1, AURA, AURKA, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46-52 kDa
GenBank Accession NumberBC002499
Gene Symbol AURKA
Gene ID (NCBI) 6790
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14965
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AURKA antibody 82906-2-RRDownload protocol
IF protocol for AURKA antibody 82906-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols