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验证数据展示

  • WB analysis using 82912-1-RR

    WB analysis using 82912-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82912-1-RR (ZC3H11A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 82912-1-RR

    IHC staining of human ovary cancer using 82912-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82912-1-RR (ZC3H11A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 82912-1-RR

    IF Staining of HepG2 using 82912-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZC3H11A antibody (82912-1-RR, Clone: 230191H5 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green).

  • Affinity and Kinetic Characterization of 82912-1-RR

    Affinity and Kinetic Characterization of 82912-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82912-1-RR against Human ZC3H11A were performed. The affinity constant is below 1 pM.

ZC3H11A Recombinant antibody

ZC3H11A Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 82912-1-RR

产品说明书

CloneNo. 230191H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Zinc finger CCCH domain-containing protein 11A, ZC3HDC11A, KIAA0663, 230191H5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, A549 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82912-1-RR targets ZC3H11A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ZC3H11A fusion protein Ag23424 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger CCCH-type containing 11A
别名 Zinc finger CCCH domain-containing protein 11A, ZC3HDC11A, KIAA0663, 230191H5
观测分子量100 kDa
GenBank蛋白编号BC014268
基因名称 ZC3H11A
Gene ID (NCBI) 9877
RRIDAB_3670644
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75152
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZC3H11A is a RNA-binding protein that interacts with purine-rich sequences and is involved in nuclear mRNA export.

实验方案

Product Specific Protocols
WB protocol for ZC3H11A antibody 82912-1-RRDownload protocol
IHC protocol for ZC3H11A antibody 82912-1-RRDownload protocol
IF protocol for ZC3H11A antibody 82912-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols