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PICK1 Recombinant antibody

PICK1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230185B8

Cat no : 82919-1-RR

Print datasheet

Synonyms

Protein kinase C-alpha-binding protein, PRKCABP, PRKCA-binding protein, PRKCA binding protein, PICK

验证数据展示

  • WB analysis using 82919-1-RR

    WB analysis using 82919-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82919-1-RR (PICK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 82919-1-RR

    WB analysis using 82919-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82919-1-RR (PICK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 82919-1-RR

    IHC staining of human stomach cancer using 82919-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HEK-293T using 82919-1-RR

    FC experiment of HEK-293T using 82919-1-RR

    1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human PICK1 (82919-1-RR, Clone:230185B8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human stomach cancer using 82919-1-RR

    IHC staining of human stomach cancer using 82919-1-RR

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 82919-1-RR

    IHC staining of human stomach cancer using 82919-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82919-1-RR

    Affinity and Kinetic Characterization of 82919-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82919-1-RR against Human PICK1 were performed. The affinity constant is 0.792 nM.

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, MCF-7 cells, mouse brain tissue, rat brain tissue, pig brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHEK-293T cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82919-1-RR targets PICK1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen PICK1 fusion protein Ag1443 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein interacting with PRKCA 1
Synonyms Protein kinase C-alpha-binding protein, PRKCABP, PRKCA-binding protein, PRKCA binding protein, PICK
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC017561
Gene Symbol PICK1
Gene ID (NCBI) 9463
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRD5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein interacting with C kinase 1 (PICK1) was first cloned as a PKC-binding partner through yeast two hybrid system. PICK1 acts as a critical regulator of membrane receptors' subcellular trafficking to modulate neural processes such as learning and memory, and is widely expressed in brain, testis, heart, lung, liver, kidney and muscle. It probably binds to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence, for instance, PICK1 is a critical mediator of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking in neural synapses. PICK1 expression on D-serine release and glutamate transport in astrocytes suggests a potential implication of PICK1 in the progression of amyotrophic lateral sclerosis (ALS). PICK1 may also participate in breast cancer development through inhibition of TGF-β signaling.

实验方案

Product Specific Protocols
WB protocol for PICK1 antibody 82919-1-RRDownload protocol
IHC protocol for PICK1 antibody 82919-1-RRDownload protocol
FC protocol for PICK1 antibody 82919-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols