• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of SH-SY5Y using 82925-2-RR

    WB analysis of SH-SY5Y using 82925-2-RR

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 82925-2-RR (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 82925-2-RR

    IF Staining of mouse brain using 82925-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STMN2 antibody (82925-2-RR, Clone: 230110B12 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 82925-2-RR

    IF Staining of SH-SY5Y using 82925-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using STMN2 antibody (82925-2-RR, Clone: 230110B12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of mouse brain using 82925-2-RR

    IHC staining of mouse brain using 82925-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82925-2-RR (STMN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STMN2 antibody included
  • IP experiment of SH-SY5Y using 82925-2-RR

    IP experiment of SH-SY5Y using 82925-2-RR

    IP result of anti-STMN2 (IP:82925-2-RR, 4ug; Detection:82925-2-RR 1:1500) with SH-SY5Y cells lysate 1240 ug.

  • FC experiment of SH-SY5Y using 82925-2-RR

    FC experiment of SH-SY5Y using 82925-2-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0 ug STMN2 Recombinant antibody (82925-2-RR, Clone:230110B12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82925-2-RR

    Affinity and Kinetic Characterization of 82925-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82925-2-RR against Human STMN2 were performed. The affinity constant is 1.48 nM.

STMN2 Recombinant antibody

STMN2 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 82925-2-RR

产品说明书

CloneNo. 230110B12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

别名

SGC 10, SCGN10, SCGN 10, SCG10, SCG 10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy STMN2 Ready-To-Use IHC Kit. STMN2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.00 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82925-2-RR targets STMN2 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 STMN2 fusion protein Ag0929 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 stathmin-like 2
别名 SGC 10, SCGN10, SCGN 10, SCG10, SCG 10
计算分子量 21 kDa
观测分子量 18-21 kDa
GenBank蛋白编号BC006302
基因名称 STMN2
Gene ID (NCBI) 11075
RRIDAB_3670662
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ93045
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)

实验方案

Product Specific Protocols
WB protocol for STMN2 antibody 82925-2-RRDownload protocol
IHC protocol for STMN2 antibody 82925-2-RRDownload protocol
IF protocol for STMN2 antibody 82925-2-RRDownload protocol
IP protocol for STMN2 antibody 82925-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols