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STMN2 Recombinant antibody

STMN2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230110G10

Cat no : 82925-3-RR

Print datasheet

Synonyms

SGC 10, SCGN10, SCGN 10, SCG10, SCG 10

验证数据展示

  • FC experiment of SH-SY5Y using 82925-3-RR

    FC experiment of SH-SY5Y using 82925-3-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug STMN2 Recombinant antibody (82925-3-RR, Clone:230110G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of SH-SY5Y using 82925-3-RR

    IF Staining of SH-SY5Y using 82925-3-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using STMN2 antibody (82925-3-RR, Clone: 230110G10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy STMN2 Ready-To-Use IHC Kit. STMN2 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82925-3-RR targets STMN2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen STMN2 fusion protein Ag0929 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name stathmin-like 2
Synonyms SGC 10, SCGN10, SCGN 10, SCG10, SCG 10
Calculated Molecular Weight 21 kDa
GenBank Accession NumberBC006302
Gene Symbol STMN2
Gene ID (NCBI) 11075
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ93045
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)

实验方案

Product Specific Protocols
IF protocol for STMN2 antibody 82925-3-RRDownload protocol
FC protocol for STMN2 antibody 82925-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols