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验证数据展示

  • FC experiment of SH-SY5Y using 82925-3-RR

    FC experiment of SH-SY5Y using 82925-3-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug STMN2 Recombinant antibody (82925-3-RR, Clone:230110G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of SH-SY5Y using 82925-3-RR

    IF Staining of SH-SY5Y using 82925-3-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using STMN2 antibody (82925-3-RR, Clone: 230110G10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

STMN2 Recombinant antibody

STMN2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 82925-3-RR

产品说明书

CloneNo. 230110G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

SGC 10, SCGN10, SCGN 10, SCG10, SCG 10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy STMN2 Ready-To-Use IHC Kit. STMN2 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82925-3-RR targets STMN2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 STMN2 fusion protein Ag0929 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 stathmin-like 2
别名 SGC 10, SCGN10, SCGN 10, SCG10, SCG 10
计算分子量 21 kDa
GenBank蛋白编号BC006302
基因名称 STMN2
Gene ID (NCBI) 11075
RRIDAB_3670663
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ93045
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)

实验方案

Product Specific Protocols
IF protocol for STMN2 antibody 82925-3-RRDownload protocol
FC protocol for STMN2 antibody 82925-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols