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验证数据展示

  • IHC staining of mouse kidney using 82954-1-RR

    IHC staining of mouse kidney using 82954-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82954-1-RR (CLDN16 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 82954-1-RR

    IHC staining of mouse kidney using 82954-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82954-1-RR (CLDN16 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 82954-1-RR

    IF Staining of HEK-293 using 82954-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CLDN16 antibody (82954-1-RR, Clone: 230193G3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CLDN16 Recombinant antibody

CLDN16 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 82954-1-RR

产品说明书

CloneNo. 230193G3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

PCLN-1, PCLN1, Paracellin-1, Claudin-16, 230193G3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82954-1-RR targets CLDN16 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CLDN16 fusion protein Ag26531 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 claudin 16
别名 PCLN-1, PCLN1, Paracellin-1, Claudin-16, 230193G3
计算分子量 305 aa, 34 kDa
GenBank蛋白编号BC069682
基因名称 CLDN16
Gene ID (NCBI) 10686
RRIDAB_3670698
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y5I7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CLDN16 antibody 82954-1-RRDownload protocol
IF protocol for CLDN16 antibody 82954-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols