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Arginase-1 Recombinant antibody

Arginase-1 Recombinant Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4M7

Cat No : 82975-1-RR

Print datasheet

Synonyms

ARG1, Arginase 1, arginase, liver, Liver type arginase, Type I arginase

验证数据展示

  • WB analysis using 82975-1-RR

    WB analysis using 82975-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82975-1-RR (Arginase-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse liver using 82975-1-RR

    IF Staining of mouse liver using 82975-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Arginase-1 antibody (82975-1-RR, Clone: 4M7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of mouse liver using 82975-1-RR

    IHC staining of mouse liver using 82975-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82975-1-RR (Arginase-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARG1 antibody included
  • Affinity experiments of 82975-1-RR

    Affinity experiments of 82975-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82975-1-RR against Human Arginase-1 were performed. The affinity constant is 4.77 nM.

经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse liver tissue
Planning an IHC experiment? We recommend our IHCeasy ARG1 Ready-To-Use IHC Kit. ARG1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82975-1-RR targets Arginase-1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen Arginase-1 fusion protein Ag8595 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name arginase, liver
Synonyms ARG1, Arginase 1, arginase, liver, Liver type arginase, Type I arginase
Calculated Molecular Weight236aa,25 kDa; 322aa,35 kDa
Observed Molecular Weight 35-36 kDa
GenBank Accession NumberBC005321
Gene Symbol ARG1
Gene ID (NCBI) 383
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05089
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). ARG1 mainly expresses in neurons in a normal brain. The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).

实验方案

Product Specific Protocols
WB protocol for Arginase-1 antibody 82975-1-RRDownload protocol
IHC protocol for Arginase-1 antibody 82975-1-RRDownload protocol
IF protocol for Arginase-1 antibody 82975-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols