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验证数据展示

  • IF Staining of HUVEC using 82993-5-RR

    IF Staining of HUVEC using 82993-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using ENG antibody (82993-5-RR, Clone: 230047E8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity and Kinetic Characterization of 82993-5-RR

    Affinity and Kinetic Characterization of 82993-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82993-5-RR against Human Endoglin/CD105 were performed. The affinity constant is 0.296 nM.

Endoglin/CD105 Recombinant antibody

Endoglin/CD105 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 82993-5-RR

产品说明书

CloneNo. 230047E8

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

IF/ICC, ELISA

别名

CD105, END, Endoglin, ENG, HHT1, ORW, ORW1, 230047E8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy Endoglin/CD105 Ready-To-Use IHC Kit. Endoglin/CD105 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82993-5-RR targets Endoglin/CD105 in IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 endoglin
别名 CD105, END, Endoglin, ENG, HHT1, ORW, ORW1, 230047E8
计算分子量 71 kDa
GenBank蛋白编号BC014271
基因名称 CD105
Gene ID (NCBI) 2022
RRIDAB_3670741
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP17813
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

实验方案

Product Specific Protocols
IF protocol for Endoglin/CD105 antibody 82993-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols