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MTAP Recombinant antibody

MTAP Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240030A3

Cat No : 83005-1-RR

Print datasheet

Synonyms

c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase

验证数据展示

  • WB analysis using 83005-1-RR

    WB analysis using 83005-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83005-1-RR (MTAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83005-1-RR

    Affinity and Kinetic Characterization of 83005-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83005-1-RR against Human MTAP were performed. The affinity constant is 0.782 nM.

经过测试的应用

Positive WB detected inHeLa cells, HCT 116 cells, HT-29 cells, NIH/3T3 cells, C6 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83005-1-RR targets MTAP in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen MTAP fusion protein Ag2051 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name methylthioadenosine phosphorylase
Synonyms c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase
Calculated Molecular Weight 283 aa, 31 kDa
Observed Molecular Weight31 kDa
GenBank Accession NumberBC018625
Gene Symbol MTAP
Gene ID (NCBI) 4507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13126
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.

实验方案

Product Specific Protocols
WB protocol for MTAP antibody 83005-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols