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验证数据展示

  • WB analysis using 83007-2-RR

    WB analysis using 83007-2-RR

    Rat heart tissue were subjected to SDS PAGE followed by western blot with 83007-2-RR (LAMA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 83007-2-RR

    WB analysis of mouse heart using 83007-2-RR

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 83007-2-RR (LAMA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83007-2-RR

    Affinity and Kinetic Characterization of 83007-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83007-2-RR against Human LAMA2 were performed. The affinity constant is below 1 pM.

LAMA2 Recombinant monoclonal antibody

LAMA2 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 83007-2-RR

产品说明书

CloneNo. 230541D5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

230541D5, Laminin subunit alpha-2, Laminin-12 subunit alpha, Laminin-2 subunit alpha, Laminin-4 subunit alpha

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat heart tissue, human placenta tissue, mouse heart tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83007-2-RR targets LAMA2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34494

Product name: Recombinant human LAMA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2100-2250 aa of NM_000426

Sequence: IDKLKPIKELEDNLKKNISEIKELINQARKQANSIKVSVSSGGDCIRTYKPEIKKGSYNNIVVNVKTAVADNLLFYLGSAKFIDFLAIEMRKGKVSFLWDVGSGVGRVEYPDLTIDDSYWYRIVASRTGRNGTISVRALDG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 laminin, alpha 2
别名 230541D5, Laminin subunit alpha-2, Laminin-12 subunit alpha, Laminin-2 subunit alpha, Laminin-4 subunit alpha
计算分子量344 kDa
观测分子量344 kDa
GenBank蛋白编号NM_000426
基因名称 LAMA2
Gene ID (NCBI) 3908
RRIDAB_3670752
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP24043
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The laminin subunit alpha 2 (LAMA2) gene encodes an alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Basal laminae are structural components of the extracellular matrix that influence cell proliferation and differentiation,7 and laminin subunit alpha 2 (LAMA2) is the major component of the basal laminae-α subunit of laminin. (PMID: 30728939)

实验方案

Product Specific Protocols
WB protocol for LAMA2 antibody 83007-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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