验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, Raji cells, HEK-293T cells, U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83074-2-RR targets PFAS in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | PFAS fusion protein Ag20162 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | phosphoribosylformylglycinamidine synthase |
| 别名 | FGAMS, FGARAT, KIAA0361, PFAS, PFAS protein, PURL |
| 计算分子量 | 1338 aa, 145 kDa |
| 观测分子量 | 140-150 kDa |
| GenBank蛋白编号 | BC167158 |
| 基因名称 | PFAS |
| Gene ID (NCBI) | 5198 |
| RRID | AB_3670793 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | O15067 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Phosphoribosylformylglycinamidine synthase (PFAS) is a highly conserved enzyme that catalyzes the fourth step of de novo purine synthesis. It catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PFAS antibody 83074-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |