p300 Recombinant antibody
p300 Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
230369H8
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | THP-1 cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83078-4-RR targets p300 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Applications | IF/ICC, FC (Intra), ELISA Application Description |
Tested Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Affinity | KD=1.30 x 10-8M KOff=3.14 x 10-3M KOn=2.42 x 105M |
Full Name | E1A binding protein p300 |
Synonyms | EP300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-, E1A-associated protein p300 |
Calculated Molecular Weight | 264 kDa |
GenBank Accession Number | NM_001429 |
Gene Symbol | EP300 |
Gene ID (NCBI) | 2033 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q09472 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IF protocol for p300 antibody 83078-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |