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验证数据展示

  • IHC staining of mouse brain using 83081-3-RR

    IHC staining of mouse brain using 83081-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83081-3-RR (STK39 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 83081-3-RR

    IF Staining of HepG2 using 83081-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STK39 antibody (83081-3-RR, Clone: 230382G12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • FC experiment of Daudi using 83081-3-RR

    FC experiment of Daudi using 83081-3-RR

    1x10^6 Daudi cells were intracellularly stained with 0.25 ug Anti-Human STK39 (83081-3-RR, Clone:230382G12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83081-3-RR

    Affinity and Kinetic Characterization of 83081-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83081-3-RR against Human STK39 were performed. The affinity constant is 1.21 nM.

STK39 Recombinant antibody

STK39 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83081-3-RR

Print datasheet

CloneNo. 230382G12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Ste 20 related kinase, SPAK, PASK, DCHT, 230382G12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inDaudi cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83081-3-RR targets STK39 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen STK39 fusion protein Ag34503 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name serine threonine kinase 39 (STE20/SPS1 homolog, yeast)
Synonyms Ste 20 related kinase, SPAK, PASK, DCHT, 230382G12
Calculated Molecular Weight59 kDa
GenBank Accession NumberBC166614
Gene Symbol STK39
Gene ID (NCBI) 27347
RRIDAB_3670798
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UEW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STK39 (Serine/Threonine Kinase 39) is a proline- and alanine-rich Ste20-related kinase (also named as SPAK), which is composed of a short N-terminal proline and alanine repeats (PAPA box), a kinase catalytic domain and a C-terminal regulatory domain (PMID: 16382158). In mammalians, STK39 plays an important role in ion homeostasis by regulating the cation chloride cotransporters' activities, which is crucial for the modulation of renal salt transport and blood pressure (PMID: 33500714). Notably, STK39 is also implicated in the regulation of tumor cell proliferation, migration, and invasion in multiple cancers, including osteosarcoma and cervical cancer (PMID: 34335956).

实验方案

Product Specific Protocols
IHC protocol for STK39 antibody 83081-3-RRDownload protocol
IF protocol for STK39 antibody 83081-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols