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验证数据展示

  • IF Staining of HepG2 using 83117-4-RR

    IF Staining of HepG2 using 83117-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using INO80 antibody (83117-4-RR, Clone: 230440E9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 83117-4-RR

    IF Staining of HepG2 using 83117-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using INO80 antibody (83117-4-RR, Clone: 230440E9 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83117-4-RR

    FC experiment of HeLa using 83117-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ino80 Recombinant Antibody (83117-4-RR, Clone:230440E9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

INO80 Recombinant antibody

INO80 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83117-4-RR

产品说明书

CloneNo. 230440E9

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

IF/ICC, FC (Intra), ELISA

别名

hINO80, INO80, INO80 homolog (S. cerevisiae), INO80A, INOC1, KIAA1259

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83117-4-RR targets INO80 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 INO80 homolog (S. cerevisiae)
别名 hINO80, INO80, INO80 homolog (S. cerevisiae), INO80A, INOC1, KIAA1259
计算分子量 1556 aa, 177 kDa
GenBank蛋白编号BC146785
基因名称 INO80
Gene ID (NCBI) 54617
RRIDAB_3670830
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9ULG1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for INO80 antibody 83117-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols