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INO80 Recombinant antibody

INO80 Recombinant Antibody for IF/ICC, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

230440E9

Cat No : 83117-4-RR

Print datasheet

Synonyms

hINO80, INO80, INO80 homolog (S. cerevisiae), INO80A, INOC1, KIAA1259

验证数据展示

  • IF Staining of HepG2 using 83117-4-RR

    IF Staining of HepG2 using 83117-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using INO80 antibody (83117-4-RR, Clone: 230440E9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 83117-4-RR

    IF Staining of HepG2 using 83117-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using INO80 antibody (83117-4-RR, Clone: 230440E9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83117-4-RR

    FC experiment of HeLa using 83117-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ino80 Recombinant Antibody (83117-4-RR, Clone:230440E9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:125-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83117-4-RR targets INO80 in IF, ELISA, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications IF/ICC, ELISA, FC (Intra) Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name INO80 homolog (S. cerevisiae)
Synonyms hINO80, INO80, INO80 homolog (S. cerevisiae), INO80A, INOC1, KIAA1259
Calculated Molecular Weight 1556 aa, 177 kDa
GenBank Accession NumberBC146785
Gene Symbol INO80
Gene ID (NCBI) 54617
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9ULG1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for INO80 antibody 83117-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols