• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83137-4-RR

    WB analysis using 83137-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83137-4-RR (RNF31 / HOIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of PC-3 using 83137-4-RR

    FC experiment of PC-3 using 83137-4-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.2 ug RNF31 / HOIP Recombinant antibody (83137-4-RR, Clone:230446E9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

RNF31 / HOIP Recombinant antibody

RNF31 / HOIP Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83137-4-RR

产品说明书

CloneNo. 230446E9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

HOIP, RNF31, HOIL 1 interacting protein, FLJ23501, FLJ10111

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, HepG2 cells
Positive FC (Intra) detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83137-4-RR targets RNF31 / HOIP in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 RNF31 / HOIP fusion protein Ag34505 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ring finger protein 31
别名 HOIP, RNF31, HOIL 1 interacting protein, FLJ23501, FLJ10111
计算分子量 1072 aa, 120 kDa
观测分子量 100 kDa
GenBank蛋白编号BC017376
基因名称 RNF31
Gene ID (NCBI) 55072
RRIDAB_3670839
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96EP0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RNF31 / HOIP antibody 83137-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols