• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PPFIA3 Recombinant antibody

PPFIA3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230459F6

Cat no : 83142-4-RR

Print datasheet

Synonyms

PTPRF-interacting protein alpha-3, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3, Liprin-alpha-3, KIAA0654, 230459F6

验证数据展示

  • WB analysis using 83142-4-RR

    WB analysis using 83142-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83142-4-RR (PPFIA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 83142-4-RR

    FC experiment of HEK-293 using 83142-4-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug PPFIA3 Recombinant antibody (83142-4-RR, Clone:230459F6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of U-251 using 83142-4-RR

    FC experiment of U-251 using 83142-4-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug PPFIA3 Recombinant antibody (83142-4-RR, Clone:230459F6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IP experiment of mouse brain using 83142-4-RR

    IP experiment of mouse brain using 83142-4-RR

    IP result of anti-PPFIA3 (IP:83142-4-RR, 4ug; Detection:83142-4-RR 1:4000) with mouse brain tissue lysate 2100 ug.

  • Affinity and Kinetic Characterization of 83142-4-RR

    Affinity and Kinetic Characterization of 83142-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83142-4-RR against Human PPFIA3 were performed. The affinity constant is 0.131 nM.

经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive FC (Intra) detected inU-251 cells, HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy PPFIA3 Ready-To-Use IHC Kit. PPFIA3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83142-4-RR targets PPFIA3 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PPFIA3 fusion protein Ag1971 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3
Synonyms PTPRF-interacting protein alpha-3, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3, Liprin-alpha-3, KIAA0654, 230459F6
Calculated Molecular Weight 1194 aa, 133 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC021255
Gene Symbol PPFIA3
Gene ID (NCBI) 8541
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75145
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPFIA3, also named as KIAA0654, belongs to the liprin family and Liprin-alpha subfamily. PPFIA3 may regulate the disassembly of focal adhesions. It may localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates. PPFIA3 exhibites more than 5 fold accumulations in the monoribosome fraction. PPFIA3 is decreased at the protein level by the miR-99 family but not in the luciferase reporter with the 3'UTR of PPFIA3. (PMID:21212412)

实验方案

Product Specific Protocols
WB protocol for PPFIA3 antibody 83142-4-RRDownload protocol
IP protocol for PPFIA3 antibody 83142-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols