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Host
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 83154-1-RR (same clone as 83154-1-PBS)

    WB analysis using 83154-1-RR (same clone as 83154-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83154-1-RR (INTS5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83154-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 83154-1-RR (same clone as 83154-1-PBS)

    IF Staining of U2OS using 83154-1-RR (same clone as 83154-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using INTS5 antibody (83154-1-RR, Clone: 230462B10 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83154-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83154-1-RR (same clone as 83154-1-PBS)

    FC experiment of HeLa using 83154-1-RR (same clone as 83154-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug INTS5 Recombinant antibody (83154-1-RR, Clone:230462B10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83154-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00141-2

    Cytometric bead array standard curve of MP00141-2

    Cytometric bead array standard curve ofMP00141-2, INTS5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83154-3-PBS. Detection antibody: 83154-1-PBS. Standard: Ag5129. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83154-1-RR

    Affinity and Kinetic Characterization of 83154-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83154-1-RR against Human INTS5 were performed. The affinity constant is 0.968 nM.

INTS5 Recombinant antibody, PBS Only (Detector)

INTS5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83154-1-PBS

产品说明书

CloneNo. 230462B10

宿主/亚型

Rabbit / IgG

种属反应性

mouse, human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

INTS 5, Int5, 230462B10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy INTS5 Ready-To-Use IHC Kit. INTS5 primary antibody included.

产品信息

83154-1-PBS targets INTS5 as part of a matched antibody pair:

MP00141-2: 83154-3-PBS capture and 83154-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse, human
免疫原 INTS5 fusion protein Ag5129 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrator complex subunit 5
别名 INTS 5, Int5, 230462B10
计算分子量 108 kDa
GenBank蛋白编号BC060841
基因名称 INTS5
Gene ID (NCBI) 80789
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6P9B9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C