• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HepG2 using 83162-7-RR

    IF Staining of HepG2 using 83162-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIC2 antibody (83162-7-RR, Clone: 230177E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 83162-7-RR

    FC experiment of HepG2 using 83162-7-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Hic2 Recombinant Antibody (83162-7-RR, Clone:230177E8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IF Staining of U2OS using 83162-7-RR

    IF Staining of U2OS using 83162-7-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HIC2 antibody (83162-7-RR, Clone: 230177E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83162-7-RR

    Affinity and Kinetic Characterization of 83162-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83162-7-RR against Human HIC2 were performed. The affinity constant is 0.527 nM.

HIC2 Recombinant antibody

HIC2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83162-7-RR

产品说明书

CloneNo. 230177E8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Hic-3, Hic-2, HIC1-related gene on chromosome 22 protein, Hic 3, Hic 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHepG2 cells, U2OS cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83162-7-RR targets HIC2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 HIC2 fusion protein Ag18789 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hypermethylated in cancer 2
别名 Hic-3, Hic-2, HIC1-related gene on chromosome 22 protein, Hic 3, Hic 2
计算分子量 615 aa, 66 kDa
GenBank蛋白编号BC094787
基因名称 HIC2
Gene ID (NCBI) 23119
RRIDAB_3670857
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96JB3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIC2 (hypermethylated in cancer 2) is a transcription factor related to the tumor suppressor HIC1, required for normal cardiac development and lost in distal variants of 22q11 Deletion Syndrome (PMID: 29061339). HIC2 is also a POK family protein, and the amino acid sequence of its BTB/POZ and zinc-finger domains are similar to those of HIC1(PMID: 31127867). Studies have shown that HIC2 plays a role in suppressing fetal genes for normal development of the heart and circulatory system, in embryogenesis (PMID: 24748541).

实验方案

Product Specific Protocols
IF protocol for HIC2 antibody 83162-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols