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验证数据展示

  • WB analysis using 83172-2-RR

    WB analysis using 83172-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83172-2-RR (USP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 83172-2-RR

    IF Staining of U2OS using 83172-2-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of U2OS using 83172-2-RR

    IF Staining of U2OS using 83172-2-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HeLa using 83172-2-RR

    IF Staining of HeLa using 83172-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A549 using 83172-2-RR

    FC experiment of A549 using 83172-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug USP8 Recombinant antibody (83172-2-RR, Clone:240016D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83172-2-RR

    Affinity and Kinetic Characterization of 83172-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83172-2-RR against Human USP8 were performed. The affinity constant is below 1 pM.

USP8 Recombinant antibody

USP8 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83172-2-RR

产品说明书

CloneNo. 240016D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

Ubiquitin carboxyl-terminal hydrolase 8, KIAA0055, HumORF8, hUBPy, EC:3.4.19.12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, K-562 cells, A549 cells, HepG2 cells, HEK-293 cells, HeLa cells, MCF-7 cells
Positive IF/ICC detected inU2OS cells, HeLa cells
Positive FC (Intra) detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy USP8 Ready-To-Use IHC Kit. USP8 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83172-2-RR targets USP8 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 USP8 fusion protein Ag27143 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin specific peptidase 8
别名 Ubiquitin carboxyl-terminal hydrolase 8, KIAA0055, HumORF8, hUBPy, EC:3.4.19.12
计算分子量 1118 aa, 128 kDa
GenBank蛋白编号BC110590
基因名称 USP8
Gene ID (NCBI) 9101
RRIDAB_3670867
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP40818
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP8 is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage.

实验方案

Product Specific Protocols
WB protocol for USP8 antibody 83172-2-RRDownload protocol
IF protocol for USP8 antibody 83172-2-RRDownload protocol
FC protocol for USP8 antibody 83172-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols