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验证数据展示

  • WB analysis using 83173-1-RR (same clone as 83173-1-PBS)

    WB analysis using 83173-1-RR (same clone as 83173-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83173-1-RR (MAP7D1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83173-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 83173-1-RR (same clone as 83173-1-PBS)

    IF Staining of U-251 using 83173-1-RR (same clone as 83173-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MAP7D1 antibody (83173-1-RR, Clone: 230424A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83173-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00212-2

    Cytometric bead array standard curve of MP00212-2

    Cytometric bead array standard curve of MP00212-2, MAP7D1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83173-1-PBS. Detection antibody: 83173-3-PBS. Standard: Ag14385. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83173-1-RR

    Affinity and Kinetic Characterization of 83173-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83173-1-RR against Human MAP7D1 were performed. The affinity constant is below 1 pM.

MAP7D1 Recombinant antibody, PBS Only (Capture)

MAP7D1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83173-1-PBS

产品说明书

CloneNo. 230424A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

PP2464, PARCC1, MAP7 domain-containing protein 1, KIAA1187, Arginine/proline-rich coiled-coil domain-containing protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83173-1-PBS targets MAP7D1 as part of a matched antibody pair:

MP00212-2: 83173-1-PBS capture and 83173-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 MAP7D1 fusion protein Ag14385 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 MAP7 domain containing 1
别名 PP2464, PARCC1, MAP7 domain-containing protein 1, KIAA1187, Arginine/proline-rich coiled-coil domain-containing protein 1
计算分子量 841 aa, 93 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC003083
基因名称 MAP7D1
Gene ID (NCBI) 55700
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ3KQU3
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAP7D1 also known as RPRC1, PARCC1, belongs to the MAP7 family. The MAP7 (Microtubule Associated Protein 7) protein family, consisting of four members, MAP7, MAP7D1, and MAP7D2, MAP7D3, is the microtubule-associated protein involved in various cellular processes regulating microtubule dynamics, organization, and stability(PMID: 28980356). MAP7D1 exhibits the highest conservation with MAP7 and was recently identified as a phosphorylation substrate of DCLK1 in cortical neurons. MAP7D1 is required to maintain MT acetylation, which is enriched in stable MTs(PMID: 35470240). Consistent with the literature, the apparent molecular mass of MAP7D1 detected by Western blot was 120-130 kDa (PMID: 35470240, 37550720).